Hotspot

trnS-GCU-trnG-UCC

LSC · 8961-9766 · 806 bp

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Pi 0.0177

MarkerSeek score 69.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 77
pi: 0.0177
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	CAAACCGAGCGATCCCTTAA	50.0	50.0	58.0	58.0	877-949 bp	1.0	90.6
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	CAAACCGAGCGATCCCTTAA	50.0	50.0	58.0	58.0	876-948 bp	1.0	90.6
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	AAACCGAGCGATCCCTTAAC	50.0	50.0	58.0	58.0	876-948 bp	1.0	90.6
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	AAACCGAGCGATCCCTTAAC	50.0	50.0	58.0	58.0	875-947 bp	1.0	90.6
trnS-GCU-trnG-UCC_p5	ACTGAACGGAAAGAGAGGGA	CAAACCGAGCGATCCCTTAA	50.0	50.0	58.0	58.0	936-1008 bp	1.0	90.1