Hotspot

psbI-trnS-GCU

LSC · 8680-8870 · 191 bp

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Pi 0.0314
MarkerSeek score 68.70
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
191
variable_sites
22
pi
0.0314
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CGTAATCCTGGACGTGAAGA TCGAATCCCTCTCTTTCCGT 50.0 50.0 57.1 58.2 233-243 bp 1.0 80.5
psbI-trnS-GCU_p2 CGTAATCCTGGACGTGAAGA TGGACTAAAGCGTCGGATTG 50.0 50.0 57.1 58.0 287-297 bp 1.0 80.0
psbI-trnS-GCU_p3 CGTAATCCTGGACGTGAAGA GTGGACTAAAGCGTCGGATT 50.0 50.0 57.1 58.0 288-298 bp 1.0 79.9
psbI-trnS-GCU_p4 CGTAATCCTGGACGTGAAGA TTTTTCGTACCGAGGGTTCG 50.0 50.0 57.1 58.2 250-260 bp 1.0 79.4
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAAGAA TGGACTAAAGCGTCGGATTG 47.6 50.0 57.8 58.0 287-297 bp 1.0 77.6