Hotspot

trnH-GUG-psbA

LSC · 78-472 · 395 bp

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Pi 0.0279

MarkerSeek score 80.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 395
variable_sites: 48
pi: 0.0279
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CAAACCTCCGCCTTAATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	264-490 bp	1.0	90.5
trnH-GUG-psbA_p2	AAACCTCCGCCTTAATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	263-489 bp	1.0	90.5
trnH-GUG-psbA_p3	ACAAACCTCCGCCTTAATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	265-491 bp	1.0	90.5
trnH-GUG-psbA_p4	CAAACCTCCGCCTTAATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	267-493 bp	1.0	90.4
trnH-GUG-psbA_p5	AAACCTCCGCCTTAATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	266-492 bp	1.0	90.4