Hotspot

rpl14

LSC · 82328-82696 · 369 bp

Back to Epidendrum

Pi 0.00813

MarkerSeek score 45.80

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 9
pi: 0.00813
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	GTAGACCGGGTCGACTGATA	AAACTGTAGCCAAAGCAGCT	55.0	45.0	58.0	58.0	1008-1098 bp	1.0	88.1
rpl14_p2	TAGACCGGGTCGACTGATAC	AAACTGTAGCCAAAGCAGCT	55.0	45.0	58.0	58.0	1007-1097 bp	1.0	88.1
rpl14_p3	TAGACCGGGTCGACTGATAC	GACAAATAGAAGCAGGGCGA	55.0	50.0	58.0	58.0	1205-1295 bp	1.0	86.8
rpl14_p4	GTAGACCGGGTCGACTGATA	TGGCGGAATGAACCAAGAAA	55.0	45.0	58.0	58.0	1573-1856 bp	1.0	85.4
rpl14_p5	TAGACCGGGTCGACTGATAC	TGGCGGAATGAACCAAGAAA	55.0	45.0	58.0	58.0	1572-1855 bp	1.0	85.4