Hotspot

rpl32-trnL-UAG

SSC · 112932-113669 · 738 bp

Back to Epidendrum

Pi 0.0344
MarkerSeek score 77.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
738
variable_sites
64
pi
0.0344
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACCTTTTGAATGGCAGTTCC TGGCGAAACCTTGGGTAAAA 45.0 45.0 56.5 57.9 1394-1499 bp 1.0 77.0
rpl32-trnL-UAG_p2 ACCTTTTGAATGGCAGTTCC ATCCGCATAAAAGAGCTGCA 45.0 45.0 56.5 58.0 1514-1619 bp 1.0 76.9
rpl32-trnL-UAG_p3 ACCTTTTGAATGGCAGTTCC TCCGCATAAAAGAGCTGCAT 45.0 45.0 56.5 58.0 1513-1618 bp 1.0 76.9
rpl32-trnL-UAG_p4 TCACCTACCTTTTGAATGGCA ATCCGCATAAAAGAGCTGCA 42.9 45.0 57.5 58.0 1520-1625 bp 1.0 76.7
rpl32-trnL-UAG_p5 TCACCTACCTTTTGAATGGCA TCCGCATAAAAGAGCTGCAT 42.9 45.0 57.5 58.0 1519-1624 bp 1.0 76.7