Hotspot

ndhF-rpl32

SSC · 111851-112757 · 907 bp

Back to Epidendrum

Pi 0.0579

MarkerSeek score 78.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 907
variable_sites: 106
pi: 0.0579
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AACCGTCCTAGTTCTACCGT	AAGTTTCGTTCTCAGTCGGG	50.0	50.0	57.8	57.8	1864-2273 bp	0.5	67.0
ndhF-rpl32_p2	AACCGTCCTAGTTCTACCGT	TTCGTTCTCAGTCGGGGTAT	50.0	50.0	57.8	58.2	1860-2269 bp	0.5	67.0
ndhF-rpl32_p3	TTTTATTGCGGGACAGCTCA	AAGTTTCGTTCTCAGTCGGG	45.0	50.0	57.8	57.8	1945-2354 bp	0.5	66.9
ndhF-rpl32_p4	TTTTATTGCGGGACAGCTCA	TTCGTTCTCAGTCGGGGTAT	45.0	50.0	57.8	58.2	1941-2350 bp	0.5	66.9
ndhF-rpl32_p5	TTTTCACTTGTTGCAACGGG	AAGTTTCGTTCTCAGTCGGG	45.0	50.0	57.7	57.8	2126 bp	0.166667	43.2