Hotspot

rps8-rpl14

LSC · 82109-82327 · 219 bp

Back to Epidendrum

Pi 0.0755

MarkerSeek score 75.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 219
variable_sites: 31
pi: 0.0755
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CGGCAATAATGTCCCTACCC	AGTTTCATTAGCACCCGAAGT	55.0	42.9	58.1	57.6	229-316 bp	1.0	79.8
rps8-rpl14_p2	CGGCAATAATGTCCCTACCC	TCATTAGCACCCGAAGTCTT	55.0	45.0	58.1	56.6	225-312 bp	1.0	79.6
rps8-rpl14_p3	CGGCAATAATGTCCCTACCC	TTCATTAGCACCCGAAGTCT	55.0	45.0	58.1	56.6	226-313 bp	1.0	79.6
rps8-rpl14_p4	CGGCAATAATGTCCCTACCC	TTCATTAGCACCCGAAGTCTT	55.0	42.9	58.1	57.3	226-313 bp	1.0	77.6
rps8-rpl14_p5	CGGCAATAATGTCCCTACCC	TTTCATTAGCACCCGAAGTCT	55.0	42.9	58.1	57.3	227-314 bp	1.0	77.6