Hotspot

rpoB-trnC-GCA

LSC · 27070-28488 · 1419 bp

Back to Epidendrum

Pi 0.0285

MarkerSeek score 71.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1419
variable_sites: 95
pi: 0.0285
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCCTACACAGGCAAATTGT	AGTATCGACAGACCTAGCCC	50.0	55.0	58.1	58.0	2280-2329 bp	0.5	62.5
rpoB-trnC-GCA_p2	CCCCTACACAGGCAAATTGT	GTATCGACAGACCTAGCCCT	50.0	55.0	58.1	58.0	2279-2328 bp	0.5	62.5
rpoB-trnC-GCA_p3	GGGCCCATATCCTTGCTTTT	AGTATCGACAGACCTAGCCC	50.0	55.0	58.2	58.0	2088-2137 bp	0.5	61.7
rpoB-trnC-GCA_p4	GGGCCCATATCCTTGCTTTT	GTATCGACAGACCTAGCCCT	50.0	55.0	58.2	58.0	2087-2136 bp	0.5	61.7
rpoB-trnC-GCA_p5	CAGAAAATACCGGATCCCCC	GTATCGACAGACCTAGCCCT	55.0	55.0	57.7	58.0	2297-2346 bp	0.5	60.9