Hotspot

trnS-GCU-trnG-UCC

LSC · 8315-9388 · 1074 bp

Back to Epidendrum

Pi 0.0312
MarkerSeek score 76.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1074
variable_sites
85
pi
0.0312
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1039-1178 bp 1.0 61.7
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1038-1177 bp 1.0 61.7
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 1042-1181 bp 1.0 60.6
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1041-1180 bp 1.0 60.6
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1040-1179 bp 1.0 60.6