Hotspot

rps16-trnQ-UUG

LSC · 5989-6892 · 904 bp

Back to Epidendrum

Pi 0.0328

MarkerSeek score 76.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 904
variable_sites: 77
pi: 0.0328
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	815-1033 bp	1.0	88.6
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	819-1037 bp	1.0	87.5
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	45.0	50.0	58.1	57.6	821-1039 bp	1.0	85.7
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	ACTCGGAGGTTCGAATCCTT	45.0	50.0	58.1	58.4	820-1038 bp	1.0	85.6
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	807-1025 bp	1.0	83.5