Hotspot

matK-trnK-UUU

LSC · 3313-4309 · 997 bp

Back to Epidendrum

Pi 0.0381

MarkerSeek score 76.80

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 997
variable_sites: 90
pi: 0.0381
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCGCGACGAGAATGGAATTT	ATCCGGGTTGCTAACTCAAC	45.0	50.0	58.0	57.9	1349-1485 bp	1.0	88.9
matK-trnK-UUU_p2	TCGCGACGAGAATGGAATTT	AACGGTAGAGTACTCGGCTT	45.0	50.0	58.0	58.2	1332-1468 bp	1.0	88.5
matK-trnK-UUU_p3	TCGCGACGAGAATGGAATTT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	58.0	58.2	1331-1467 bp	1.0	88.5
matK-trnK-UUU_p4	TCGCGACGAGAATGGAATTT	TCTATCCCAATGGGCCATCT	45.0	50.0	58.0	57.9	2078-2159 bp	1.0	88.3
matK-trnK-UUU_p5	TCGCGACGAGAATGGAATTT	TCGATCCCGAAGAGAAGGAA	45.0	50.0	58.0	57.9	2036-2117 bp	1.0	88.3