Hotspot

ycf3

Genome · 14189-15978 · 1790 bp

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Pi 0.00545

MarkerSeek score 59.70

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: Genome
length_bp: 1790
variable_sites: 60
pi: 0.00545
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	CCTGGGGTGGACATAATTGG	CATTCCAAAAGGAACTGAAGCA	55.0	40.9	57.9	57.4	1929-1964 bp	1.0	64.6
ycf3_p2	CCTGGGGTGGACATAATTGG	TCCAAAAGGAACTGAAGCAA	55.0	40.0	57.9	55.4	1926-1961 bp	1.0	64.4
ycf3_p3	CCTGGGGTGGACATAATTGG	TTCCAAAAGGAACTGAAGCA	55.0	40.0	57.9	55.4	1927-1962 bp	1.0	64.3
ycf3_p4	CCTGGGGTGGACATAATTGG	TCCAAAAGGAACTGAAGCAAA	55.0	38.1	57.9	56.2	1926-1961 bp	1.0	62.8
ycf3_p5	CCTGGGGTGGACATAATTGG	TTCCAAAAGGAACTGAAGCAA	55.0	38.1	57.9	56.2	1927-1962 bp	1.0	62.8