Hotspot

trnL-AUG-ycf1

Genome · 82455-82850 · 396 bp

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Pi 0.0192

MarkerSeek score 52.90

Primer available yes

Label trnL-AUG-ycf1

Feature Metadata

label_name: trnL-AUG-ycf1
feature_type: igs
region: Genome
length_bp: 396
variable_sites: 31
pi: 0.0192
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-AUG-ycf1_p1	TTGCACCCAACCAACGAATA	CGGCTGATGGAAGTCGTAAT	45.0	50.0	58.0	57.8	879-914 bp	1.0	86.1
trnL-AUG-ycf1_p2	TAATTGCACCCAACCAACGA	CGGCTGATGGAAGTCGTAAT	45.0	50.0	58.0	57.8	882-917 bp	1.0	86.0
trnL-AUG-ycf1_p3	AGAGCAGCGTGTCTACCTAT	CGGCTGATGGAAGTCGTAAT	50.0	50.0	57.9	57.8	468-504 bp	0.971429	85.9
trnL-AUG-ycf1_p4	GTAATTGCACCCAACCAACG	CGGCTGATGGAAGTCGTAAT	50.0	50.0	57.9	57.8	883-918 bp	1.0	85.7
trnL-AUG-ycf1_p5	CCGTGAGTAAACATAGCGCT	CGGCTGATGGAAGTCGTAAT	50.0	50.0	58.1	57.8	910-941 bp	0.771429	75.3