Hotspot

psaC-ccsA

Genome · 81284-81389 · 106 bp

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Pi 0.0412

MarkerSeek score 66.10

Primer available yes

Label psaC-ccsA

Feature Metadata

label_name: psaC-ccsA
feature_type: igs
region: Genome
length_bp: 106
variable_sites: 16
pi: 0.0412
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ccsA_p1	ACCACTCGTAGTATGGGACT	TGTTTGGATATGCTATTTTGGTGT	50.0	33.3	57.2	57.2	205-235 bp	1.0	50.4
psaC-ccsA_p2	AACCACTCGTAGTATGGGACT	TGTTTGGATATGCTATTTTGGTGT	47.6	33.3	57.9	57.2	206-236 bp	1.0	50.3
psaC-ccsA_p3	CGAAACCACTCGTAGTATGGG	TGTTTGGATATGCTATTTTGGTGT	52.4	33.3	58.2	57.2	209-239 bp	1.0	50.3
psaC-ccsA_p4	ACCACTCGTAGTATGGGACT	TTGTTTGGATATGCTATTTTGGTGT	50.0	32.0	57.2	57.8	206-236 bp	1.0	50.3
psaC-ccsA_p5	ACCACTCGTAGTATGGGACT	TGTTTGGATATGCTATTTTGGTGTT	50.0	32.0	57.2	57.8	215 bp	0.085714	4.5