Hotspot

clpP-psbB

Genome · 47520-48695 · 1176 bp

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Pi 0.0074

MarkerSeek score 59.00

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: Genome
length_bp: 1176
variable_sites: 68
pi: 0.0074
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TCAACGGGTTGCCCTAAAAA	GCTCCGAATCCAAAGCAAAC	45.0	50.0	57.9	58.0	1671-1724 bp	1.0	85.1
clpP-psbB_p2	TGGTTCCCACATTGGAAGTG	GCTCCGAATCCAAAGCAAAC	50.0	50.0	58.0	58.0	2164-2217 bp	1.0	84.7
clpP-psbB_p3	TGGTTCCCACATTGGAAGTG	ATGATGCGACGCTATTCCTC	50.0	50.0	58.0	57.9	2306-2359 bp	1.0	84.0
clpP-psbB_p4	GGCCAAGACCGATTGTATGT	GCTCCGAATCCAAAGCAAAC	50.0	50.0	58.0	58.0	1856-1909 bp	0.971429	83.8
clpP-psbB_p5	AACATGGTTCGGGATTAGCC	GCTCCGAATCCAAAGCAAAC	50.0	50.0	57.9	58.0	2236-2289 bp	0.971429	83.0