Hotspot

trnF-GAA-trnfM-CAU

Genome · 35590-35941 · 352 bp

Back to Ephedra

Pi 0.0105
MarkerSeek score 49.80
Primer available yes
Label trnF-GAA-trnfM-CAU

Feature Metadata

label_name
trnF-GAA-trnfM-CAU
feature_type
igs
region
Genome
length_bp
352
variable_sites
24
pi
0.0105
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-trnfM-CAU_p1 CCGGGATAGCTCAGTTGGTA ACTCTCGCCTTATGAAAGCG 55.0 50.0 58.6 58.1 471-671 bp 1.0 78.4
trnF-GAA-trnfM-CAU_p2 TCAGTTGGTAGAGCAGAGGA ACTCTCGCCTTATGAAAGCG 50.0 50.0 57.4 58.1 461-661 bp 1.0 78.3
trnF-GAA-trnfM-CAU_p3 TGGTAGAGCAGAGGACTGAA ACTCTCGCCTTATGAAAGCG 50.0 50.0 57.4 58.1 456-656 bp 1.0 78.3
trnF-GAA-trnfM-CAU_p4 TTGGTAGAGCAGAGGACTGA ACTCTCGCCTTATGAAAGCG 50.0 50.0 57.4 58.1 457-657 bp 1.0 78.3
trnF-GAA-trnfM-CAU_p5 AGTTGGTAGAGCAGAGGACT ACTCTCGCCTTATGAAAGCG 50.0 50.0 57.4 58.1 459-659 bp 1.0 78.3