Hotspot

rps4-psbM

Genome · 16877-17204 · 328 bp

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Pi 0.0139

MarkerSeek score 47.80

Primer available yes

Label rps4-psbM

Feature Metadata

label_name: rps4-psbM
feature_type: igs
region: Genome
length_bp: 328
variable_sites: 16
pi: 0.0139
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-psbM_p1	CTGTTGACCCCTGAGCATTT	AAGTGGTAAGGCAGAGGACT	50.0	50.0	58.1	58.0	1044-1075 bp	1.0	86.1
rps4-psbM_p2	TGCCGTAATTGTCGTTCTGT	AAGTGGTAAGGCAGAGGACT	45.0	50.0	57.8	58.0	1006-1037 bp	1.0	85.8
rps4-psbM_p3	CTGTTGACCCCTGAGCATTT	CAAGTGGTAAGGCAGAGGAC	50.0	55.0	58.1	57.9	1045-1076 bp	1.0	85.5
rps4-psbM_p4	CAAATCCCTCTCTTTCCGCA	AAGTGGTAAGGCAGAGGACT	50.0	50.0	57.9	58.0	1492-1528 bp	1.0	84.3
rps4-psbM_p5	CTGTTGACCCCTGAGCATTT	CTGGGTGTCGCCTACTTTTT	50.0	50.0	58.1	58.1	997-1028 bp	0.971429	83.9