Hotspot

psbZ-trnG-UCC

Genome · 8556-8703 · 148 bp

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Pi 0.0121

MarkerSeek score 59.70

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: Genome
length_bp: 148
variable_sites: 12
pi: 0.0121
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTTCGCTTTCCCAGAAGGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	288-372 bp	1.0	85.2
psbZ-trnG-UCC_p2	CTTTCGCTTTCCCAGAAGGT	TGAGGTCACGGGTTCAAATC	50.0	50.0	58.1	57.8	360-444 bp	1.0	84.9
psbZ-trnG-UCC_p3	CTTTCGCTTTCCCAGAAGGT	ATGGTTATTTTGTGGGCGGA	50.0	45.0	58.1	57.8	326-410 bp	0.971429	83.5
psbZ-trnG-UCC_p4	CTTTCGCTTTCCCAGAAGGT	TGGTTATTTTGTGGGCGGAT	50.0	45.0	58.1	57.8	325-409 bp	0.971429	83.5
psbZ-trnG-UCC_p5	ACCTATCGCTTTCGCTTTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	296-380 bp	1.0	83.1