Hotspot

ccsA-ndhD

SSC · 106803-106996 · 194 bp

Back to Enteropogon

Pi 0.0881

MarkerSeek score 71.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 194
variable_sites: 17
pi: 0.0881
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAACTCTTTTGTGCGGATC	TCTGGACCACGAGAACTCTT	50.0	50.0	58.0	58.0	856-857 bp	1.0	82.5
ccsA-ndhD_p2	GCAACTCTTTTGTGCGGATC	TTCTGGACCACGAGAACTCT	50.0	50.0	58.0	58.0	857-858 bp	1.0	82.5
ccsA-ndhD_p3	GCAACTCTTTTGTGCGGATC	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	860-861 bp	1.0	82.4
ccsA-ndhD_p4	ACCTGCTCTCCAATCTCAGT	TCTGGACCACGAGAACTCTT	50.0	50.0	58.1	58.0	914-915 bp	1.0	82.2
ccsA-ndhD_p5	ACCTGCTCTCCAATCTCAGT	TTCTGGACCACGAGAACTCT	50.0	50.0	58.1	58.0	915-916 bp	1.0	82.2