Hotspot

rpl33-rps18

LSC · 65822-66106 · 285 bp

Back to Enteropogon

Pi 0.0551

MarkerSeek score 70.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 285
variable_sites: 14
pi: 0.0551
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGAATCGCCACAATACACCC	ACCAATTCGAGAACGCCTAC	50.0	50.0	58.0	58.0	499-527 bp	1.0	86.1
rpl33-rps18_p2	AGAATCGCCACAATACACCC	TCGAGAACGCCTACGAAAAG	50.0	50.0	58.0	58.0	493-521 bp	1.0	86.0
rpl33-rps18_p3	AGAATCGCCACAATACACCC	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.0	58.0	700-728 bp	1.0	85.1
rpl33-rps18_p4	AAAGGAGGTTCATGGCCAAG	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.1	58.0	820-848 bp	1.0	84.1
rpl33-rps18_p5	GAAAGGAGGTTCATGGCCAA	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.1	58.0	821-849 bp	1.0	84.1