Hotspot

ndhC-trnV-UAC

LSC · 50654-51589 · 936 bp

Back to Enteropogon

Pi 0.0586
MarkerSeek score 80.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
936
variable_sites
54
pi
0.0586
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGGAACA GGTTCGAATCCGTATAGCCC 40.9 55.0 57.8 57.9 976-981 bp 1.0 67.4
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGGAACA CGGTTCGAATCCGTATAGCC 40.9 55.0 57.8 58.3 977-982 bp 1.0 65.6
ndhC-trnV-UAC_p3 CATATTCGTGAAGCAGGAACA GGTTCGAATCCGTATAGCCC 42.9 55.0 56.3 57.9 975-980 bp 1.0 63.8
ndhC-trnV-UAC_p4 CATATTCGTGAAGCAGGAACA CGGTTCGAATCCGTATAGCC 42.9 55.0 56.3 58.3 976-981 bp 1.0 62.0
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGGAAC GGTTCGAATCCGTATAGCCC 42.9 55.0 56.1 57.9 976-981 bp 1.0 61.6