Hotspot

trnG-UCC-trnT-GGU

LSC · 13801-14808 · 1008 bp

Back to Enteropogon

Pi 0.0305

MarkerSeek score 66.30

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1008
variable_sites: 30
pi: 0.0305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	1081-1098 bp	1.0	44.8
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	38.1	47.6	55.5	57.5	1077-1094 bp	1.0	44.1
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	ACCATGGCATTACTCTACCAC	38.1	47.6	55.5	57.4	1060-1077 bp	1.0	44.1
trnG-UCC-trnT-GGU_p4	ACGAATCACACTTTTACCACT	ACCGATGACTTATGCCTTACC	38.1	47.6	55.5	57.5	1078-1095 bp	1.0	44.0
trnG-UCC-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	39.1	45.0	57.3	56.6	1083-1100 bp	1.0	44.0