Hotspot

trnfM-CAU-trnG-UCC

LSC · 12960-13055 · 96 bp

Back to Enteropogon

Pi 0.061
MarkerSeek score 73.80
Primer available yes
Label trnfM-CAU-trnG-UCC

Feature Metadata

label_name
trnfM-CAU-trnG-UCC
feature_type
igs
region
LSC
length_bp
96
variable_sites
5
pi
0.061
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnG-UCC_p1 CCTCAAGGTTATGAGCCTCG AGCCTTCCAAGCTAACGATG 55.0 50.0 57.8 58.0 173-187 bp 1.0 84.1
trnfM-CAU-trnG-UCC_p2 CCTCAAGGTTATGAGCCTCG CCTAGCCTTCCAAGCTAACG 55.0 55.0 57.8 58.1 176-190 bp 1.0 83.9
trnfM-CAU-trnG-UCC_p3 CCTCGTGAGCTACCAAACTG AGCCTTCCAAGCTAACGATG 55.0 50.0 58.3 58.0 158-172 bp 1.0 83.9
trnfM-CAU-trnG-UCC_p4 CCTCGTGAGCTACCAAACTG CCTAGCCTTCCAAGCTAACG 55.0 55.0 58.3 58.1 161-175 bp 1.0 83.6
trnfM-CAU-trnG-UCC_p5 CCTCAAGGTTATGAGCCTCG CCAACGTCGACTATAACCCC 55.0 55.0 57.8 57.8 194-208 bp 1.0 82.4