Hotspot

ccsA-ndhD

SSC · 119811-120120 · 310 bp

Back to Entandrophragma

Pi 0.0349

MarkerSeek score 67.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 310
variable_sites: 21
pi: 0.0349
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGGGTCAATCTATTAGGAATAGGG	AGTGGACAAGGTCGAAGCTA	45.8	50.0	57.7	58.4	404-408 bp	1.0	47.4
ccsA-ndhD_p2	GGGGTCAATCTATTAGGAATAGGG	GTGGACAAGGTCGAAGCTAT	45.8	50.0	57.7	57.3	403-407 bp	1.0	45.0
ccsA-ndhD_p3	GGGGTCAATCTATTAGGAATAGGG	TCCGGATTTCGTTCTCTCAC	45.8	50.0	57.7	57.4	428-432 bp	1.0	44.9
ccsA-ndhD_p4	GGGGTCAATCTATTAGGAATAGGG	CCGGATTTCGTTCTCTCACT	45.8	50.0	57.7	57.3	427-431 bp	1.0	44.9
ccsA-ndhD_p5	GGGGTCAATCTATTAGGAATAGGG	ATCAGTGGACAAGGTCGAAG	45.8	50.0	57.7	57.2	407-411 bp	1.0	44.9