Hotspot

trnR-trnN

IRb · 112071-112682 · 612 bp

Back to Entandrophragma

Pi 0.0079

MarkerSeek score 64.30

Primer available yes

Label trnR-trnN

Feature Metadata

label_name: trnR-trnN
feature_type: igs
region: IRb
length_bp: 612
variable_sites: 9
pi: 0.0079
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnN_p1	TAGGGTACTTCCGGGAACAG	TGGGTTGAGAACGGGAATTG	55.0	50.0	58.1	58.1	845-847 bp	1.0	79.0
trnR-trnN_p2	TAGGGTACTTCCGGGAACAG	GCTGTTAACTGACTGGTCGT	55.0	50.0	58.1	57.8	768-770 bp	1.0	78.7
trnR-trnN_p3	TAGGGTACTTCCGGGAACAG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	58.1	58.2	755-757 bp	1.0	78.7
trnR-trnN_p4	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	794-796 bp	1.0	78.5
trnR-trnN_p5	TAGGGTACTTCCGGGAACAG	GGGTTGAGAACGGGAATTGA	55.0	50.0	58.1	57.8	844-846 bp	1.0	78.2