Hotspot

rpl14-rpl16

LSC · 84634-84763 · 130 bp

Back to Entandrophragma

Pi 0.0205

MarkerSeek score 71.90

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 130
variable_sites: 5
pi: 0.0205
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GGCGATTACTGGCTCCTATG	CAATAGCGGCGTCCAAAATG	55.0	50.0	57.9	58.2	264-275 bp	1.0	82.9
rpl14-rpl16_p2	GGCGATTACTGGCTCCTATG	TCAATAGCGGCGTCCAAAAT	55.0	45.0	57.9	58.3	265-276 bp	1.0	82.3
rpl14-rpl16_p3	GGCGATTACTGGCTCCTATG	ATTTCAATAGCGGCGTCCAA	55.0	45.0	57.9	58.3	268-279 bp	1.0	82.2
rpl14-rpl16_p4	TACACATCAATTCTCGGGCC	CAATAGCGGCGTCCAAAATG	50.0	50.0	57.7	58.2	237-248 bp	1.0	81.6
rpl14-rpl16_p5	CATATCGGCGATTACTGGCT	CAATAGCGGCGTCCAAAATG	50.0	50.0	57.6	58.2	270-281 bp	1.0	80.6