Hotspot

psbE-petL

LSC · 67947-69228 · 1282 bp

Back to Entandrophragma

Pi 0.00652

MarkerSeek score 64.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1282
variable_sites: 16
pi: 0.00652
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GACCAATAAATAGAGCCGAGGT	50.0	45.5	57.9	57.7	1449-1479 bp	1.0	62.7
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GACCAATAAATAGAGCCGAGGT	50.0	45.5	57.8	57.7	1372-1402 bp	1.0	62.0
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GACCAATAAATAGAGCCGAGGT	45.0	45.5	58.2	57.7	1458-1488 bp	1.0	61.8
psbE-petL_p4	TGCTGACGAATAACCAACCC	GACCAATAAATAGAGCCGAGGT	50.0	45.5	58.2	57.7	1463-1493 bp	1.0	61.7
psbE-petL_p5	CGAATAACCAACCCGCAATG	GACCAATAAATAGAGCCGAGGT	50.0	45.5	57.8	57.7	1457-1487 bp	1.0	61.6