Hotspot

petA-psbJ

LSC · 66094-67161 · 1068 bp

Back to Entandrophragma

Pi 0.00968

MarkerSeek score 53.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1068
variable_sites: 18
pi: 0.00968
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCGAATGGACGTGAAGTGG	GTCTCTAGCACGACCACTTG	50.0	55.0	58.0	58.0	1489-1507 bp	1.0	81.9
petA-psbJ_p2	ATGCATCGAATGGACGTGAA	GTCTCTAGCACGACCACTTG	45.0	55.0	58.0	58.0	1493-1511 bp	1.0	81.8
petA-psbJ_p3	ATCGAATGGACGTGAAGTGG	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1699-1717 bp	1.0	81.4
petA-psbJ_p4	CTTGTGATAGGGCCTGTTCC	GTCTCTAGCACGACCACTTG	55.0	55.0	58.0	58.0	1743-1761 bp	1.0	81.4
petA-psbJ_p5	ATCGAATGGACGTGAAGTGG	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	1932-1950 bp	1.0	81.3