Hotspot

psaA-ycf3

LSC · 44421-45068 · 648 bp

Back to Entandrophragma

Pi 0.00617

MarkerSeek score 64.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 8
pi: 0.00617
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	761-768 bp	1.0	80.1
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	753-760 bp	1.0	79.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCTATTGCGCTTACTCCTG	50.0	50.0	57.9	57.8	794-801 bp	1.0	79.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TGAAGCGCAGAATTGGTTGA	50.0	45.0	57.9	58.4	764-771 bp	1.0	77.8
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TTGAAGCGCAGAATTGGTTG	50.0	45.0	57.9	57.6	765-772 bp	1.0	77.6