Hotspot

atpF-atpH

LSC · 14327-14789 · 463 bp

Back to Entandrophragma

Pi 0.0253

MarkerSeek score 74.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 463
variable_sites: 23
pi: 0.0253
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GCCAGTGAACCAAAGAAACG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	617-620 bp	1.0	77.3
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	643-646 bp	1.0	77.2
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GAGGCGGAGGGAAAAATACG	50.0	55.0	57.9	58.7	647-650 bp	1.0	77.0
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATACGAGG	50.0	55.0	57.9	58.7	644-647 bp	1.0	77.0
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GGCGGAGGGAAAAATACGAG	50.0	55.0	57.9	58.7	645-648 bp	1.0	77.0