Hotspot

rps16-trnQ

LSC · 6453-8080 · 1628 bp

Back to Entandrophragma

Pi 0.00842

MarkerSeek score 55.50

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1628
variable_sites: 27
pi: 0.00842
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1755-1779 bp	1.0	81.5
rps16-trnQ_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1697-1721 bp	1.0	81.1
rps16-trnQ_p3	AAGTCGCACGTTGCTTTCTA	CGAAATTGAAATGGGGCGTG	45.0	50.0	58.1	58.4	1756-1780 bp	1.0	78.6
rps16-trnQ_p4	AAGTCGCACGTTGCTTTCTA	TCGAAATTGAAATGGGGCGT	45.0	45.0	58.1	58.5	1757-1781 bp	1.0	77.9
rps16-trnQ_p5	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	1718-1742 bp	1.0	77.4