Hotspot

ndhF

SSC · 122506-124719 · 2214 bp

Pi 0.0195

MarkerSeek score 56.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 81
pi: 0.0195
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CAGTGGACGGTTAGGAACAC	AGCTCAGAGTGCTATGTTTGT	55.0	42.9	58.2	57.3	2810-2854 bp	1.0	70.0
ndhF_p2	CAGTGGACGGTTAGGAACAC	GAGTGTCCTCCGATGAACAT	55.0	50.0	58.2	57.0	2673-2727 bp	0.75	65.1
ndhF_p3	GGACGGTTAGGAACACCAAA	GAGTGTCCTCCGATGAACAT	50.0	50.0	57.7	57.0	2669-2723 bp	0.75	64.7
ndhF_p4	TCAGTGGACGGTTAGGAACA	GAGTGTCCTCCGATGAACAT	50.0	50.0	58.3	57.0	2674-2728 bp	0.75	64.4
ndhF_p5	TGGACGGTTAGGAACACCAA	GAGTGTCCTCCGATGAACAT	50.0	50.0	58.9	57.0	2670-2724 bp	0.75	59.8