Hotspot

ndhA

SSC · 132467-134657 · 2191 bp

Pi 0.0161

MarkerSeek score 56.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2191
variable_sites: 63
pi: 0.0161
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACACGAACACATACTTCGCA	GTCCACCCGGCTTCATAAAT	45.0	50.0	57.9	57.9	2592-2620 bp	1.0	82.1
ndhA_p2	ACACGAACACATACTTCGCA	ACGAATCGGTGAAATGAGGG	45.0	50.0	57.9	58.0	2867-2895 bp	1.0	82.0
ndhA_p3	CGAACACATACTTCGCAAGC	ACGAATCGGTGAAATGAGGG	50.0	50.0	57.9	58.0	2864-2892 bp	1.0	82.0
ndhA_p4	CGAACACATACTTCGCAAGC	GTCCACCCGGCTTCATAAAT	50.0	50.0	57.9	57.9	2589-2617 bp	1.0	82.0
ndhA_p5	ACCTTGCAGCTCGTATTGTT	GGGCATTATTGGTGGAGAGG	45.0	55.0	57.8	58.0	2881-2909 bp	1.0	81.7