Hotspot

ccsA-ndhD

SSC · 127676-127908 · 233 bp

Pi 0.0568

MarkerSeek score 78.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 233
variable_sites: 22
pi: 0.0568
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGGACCCCAAAGAAACTTG	TTGCATTGCCAGGAATGAGT	50.0	45.0	57.9	58.1	796-811 bp	1.0	84.6
ccsA-ndhD_p2	TGGGACCCCAAAGAAACTTG	AAAATGGGCGCATATGGACT	50.0	45.0	57.9	57.9	1190-1205 bp	1.0	83.2
ccsA-ndhD_p3	TGGGACCCCAAAGAAACTTG	AAATGGGCGCATATGGACTT	50.0	45.0	57.9	57.9	1189-1204 bp	1.0	83.2
ccsA-ndhD_p4	TGGGACCCCAAAGAAACTTG	GGATTCTGGACCACGAGAAC	50.0	55.0	57.9	58.0	588-603 bp	0.75	74.1
ccsA-ndhD_p5	TTGGACCATATTCGCGGTTT	GGATTCTGGACCACGAGAAC	45.0	55.0	57.9	58.0	571 bp	0.25	49.0