Hotspot

rpl32-trnL-UAG

SSC · 125694-126501 · 808 bp

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Pi 0.0421
MarkerSeek score 77.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
808
variable_sites
62
pi
0.0421
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGAATGGCAGTCCCCAAAAA GTCCCGAAGAAATCCAACGA 45.0 50.0 57.9 57.9 1318-1368 bp 1.0 86.1
rpl32-trnL-UAG_p2 TTGAATGGCAGTCCCCAAAA GTCCCGAAGAAATCCAACGA 45.0 50.0 57.9 57.9 1319-1369 bp 1.0 86.1
rpl32-trnL-UAG_p3 TTTGAATGGCAGTCCCCAAA GTCCCGAAGAAATCCAACGA 45.0 50.0 57.9 57.9 1320-1370 bp 1.0 86.1
rpl32-trnL-UAG_p4 TTTTGAATGGCAGTCCCCAA GTCCCGAAGAAATCCAACGA 45.0 50.0 57.9 57.9 1321-1371 bp 1.0 86.1
rpl32-trnL-UAG_p5 TTTTTGAATGGCAGTCCCCA GTCCCGAAGAAATCCAACGA 45.0 50.0 57.9 57.9 1322-1372 bp 1.0 86.1