Hotspot

ndhA-ndhF

IRb · 121991-122505 · 515 bp

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Pi 0.0252
MarkerSeek score 63.60
Primer available yes
Label ndhA-ndhF

Feature Metadata

label_name
ndhA-ndhF
feature_type
igs
region
IRb
length_bp
515
variable_sites
22
pi
0.0252
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA-ndhF_p1 CGCCTCATTTTAGCCGATCT CACTTCCATGTACCCTCACG 50.0 55.0 58.1 58.0 1312-1367 bp 1.0 86.0
ndhA-ndhF_p2 TGCAGCGATACAACAACGTA CACTTCCATGTACCCTCACG 45.0 55.0 57.9 58.0 1508-1563 bp 1.0 85.6
ndhA-ndhF_p3 TGCAGCGATACAACAACGTA ACTTCCATGTACCCTCACGA 45.0 50.0 57.9 58.1 1507-1562 bp 1.0 85.1
ndhA-ndhF_p4 GTCCACCCGGCTTCATAAAT CACTTCCATGTACCCTCACG 50.0 55.0 57.9 58.0 1792-1847 bp 1.0 84.7
ndhA-ndhF_p5 GTCCACCCGGCTTCATAAAT ACTTCCATGTACCCTCACGA 50.0 50.0 57.9 58.1 1791-1846 bp 1.0 84.3