Hotspot

rps8-rpl14

LSC · 83896-84124 · 229 bp

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Pi 0.0443
MarkerSeek score 73.10
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
229
variable_sites
17
pi
0.0443
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 CGGCAATAGTGTCCTTACCC TCATTGGCTCCCGAGGTATT 55.0 50.0 58.1 58.5 272-277 bp 1.0 84.6
rps8-rpl14_p2 CGGCAATAGTGTCCTTACCC TTCATTGGCTCCCGAGGTAT 55.0 50.0 58.1 58.5 273-278 bp 1.0 84.6
rps8-rpl14_p3 CGGCAATAGTGTCCTTACCC TTTCATTGGCTCCCGAGGTA 55.0 50.0 58.1 58.7 274-279 bp 1.0 82.8
rps8-rpl14_p4 CGGCAATAGTGTCCTTACCC TTCATTGGCTCCCGAGGTA 55.0 52.6 58.1 58.0 273-278 bp 1.0 80.5
rps8-rpl14_p5 TGCTATTCGAACCGTTCCTT TCATTGGCTCCCGAGGTATT 45.0 50.0 57.2 58.5 331-336 bp 1.0 78.1