Hotspot

ycf4-cemA

LSC · 63283-63952 · 670 bp

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Pi 0.0382
MarkerSeek score 75.20
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
670
variable_sites
42
pi
0.0382
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGCGCGTACCAATTGAAGTA AGATGCGAGATATGGGAGGG 45.0 55.0 57.9 58.4 591-741 bp 1.0 84.7
ycf4-cemA_p2 TGCGCGTACCAATTGAAGTA ATGCGAGATATGGGAGGGAG 45.0 55.0 57.9 58.4 589-739 bp 1.0 84.7
ycf4-cemA_p3 TGCGCGTACCAATTGAAGTA GATGCGAGATATGGGAGGGA 45.0 55.0 57.9 58.4 590-740 bp 1.0 84.7
ycf4-cemA_p4 CTTGCGCGTACCAATTGAAG AGATGCGAGATATGGGAGGG 50.0 55.0 58.1 58.4 593-743 bp 1.0 84.4
ycf4-cemA_p5 CTTGCGCGTACCAATTGAAG ATGCGAGATATGGGAGGGAG 50.0 55.0 58.1 58.4 591-741 bp 1.0 84.4