Hotspot

atpB-rbcL

LSC · 57320-58176 · 857 bp

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Pi 0.0113
MarkerSeek score 63.50
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
857
variable_sites
18
pi
0.0113
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CGATACGCCCTAGGTTTTGT GGTGACATAAGTCCCTCCCT 50.0 55.0 57.7 58.1 934-972 bp 1.0 80.2
atpB-rbcL_p2 GCAGGACTAGAAGGGGTAGG GGTGACATAAGTCCCTCCCT 60.0 55.0 58.6 58.1 896-934 bp 1.0 78.1
atpB-rbcL_p3 CGATACGCCCTAGGTTTTGT TGGTGACATAAGTCCCTCCC 50.0 55.0 57.7 58.4 935-973 bp 1.0 77.8
atpB-rbcL_p4 ACGCCCTAGGTTTTGTTCTT GGTGACATAAGTCCCTCCCT 45.0 55.0 57.3 58.1 930-968 bp 1.0 77.5
atpB-rbcL_p5 TCTTCAAGTGTGGAAACCGC GGTGACATAAGTCCCTCCCT 50.0 55.0 58.7 58.1 914-952 bp 1.0 77.2