Hotspot

tRNA-Lys-rps16

LSC · 4279-5019 · 741 bp

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Pi 0.0265
MarkerSeek score 65.70
Primer available yes
Label tRNA-Lys-rps16

Feature Metadata

label_name
tRNA-Lys-rps16
feature_type
igs
region
LSC
length_bp
741
variable_sites
37
pi
0.0265
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Lys-rps16_p1 AGAACTCACCCTTTCCCGTA TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.0 57.9 1364-1382 bp 1.0 84.0
tRNA-Lys-rps16_p2 ACTCACCCTTTCCCGTATCA TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.1 57.9 1361-1379 bp 1.0 83.8
tRNA-Lys-rps16_p3 AGAACTCACCCTTTCCCGTA AAGAGGTTAGAGACGGCTCA 50.0 50.0 58.0 57.8 1682-1710 bp 1.0 82.7
tRNA-Lys-rps16_p4 AGAACTCACCCTTTCCCGTA AGAGGTTAGAGACGGCTCAA 50.0 50.0 58.0 57.8 1681-1709 bp 1.0 82.7
tRNA-Lys-rps16_p5 TGAGGATCAGTCGTGGTCTT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.1 57.9 1040-1049 bp 1.0 82.5