Hotspot

petA

LSC · 57985-58947 · 963 bp

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Pi 0.00104

MarkerSeek score 57.60

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AATTCCGGGATCCACGATTG	TTATTGTTCTCGCCTCTGCC	50.0	50.0	58.0	58.0	1099 bp	1.0	79.6
petA_p2	GAAATTCCGGGATCCACGAT	TTATTGTTCTCGCCTCTGCC	50.0	50.0	57.7	58.0	1101 bp	1.0	77.8
petA_p3	AATTCCGGGATCCACGATTG	CCTCTGCCTTGGATTCTGTT	50.0	50.0	58.0	57.5	1087 bp	1.0	75.8
petA_p4	AAATTCCGGGATCCACGATT	TTATTGTTCTCGCCTCTGCC	45.0	50.0	57.3	58.0	1100 bp	1.0	74.0
petA_p5	GAAATTCCGGGATCCACGAT	CCTCTGCCTTGGATTCTGTT	50.0	50.0	57.7	57.5	1089 bp	1.0	74.0