Hotspot

ndhG-ndhI

SSC · 108122-108336 · 215 bp

Back to Enneapogon

Pi 0.0233

MarkerSeek score 65.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 215
variable_sites: 5
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCAGCGAAAAGGCAGAATA	AGTCGGTTACCAGTCTCCAT	50.0	50.0	58.2	57.8	451 bp	1.0	77.0
ndhG-ndhI_p2	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAGTCTCCA	50.0	55.0	58.2	58.5	452 bp	1.0	75.2
ndhG-ndhI_p3	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAGTCTCC	50.0	55.0	58.2	58.5	453 bp	1.0	75.2
ndhG-ndhI_p4	CCCAGCGAAAAGGCAGAATA	AGTCGGTTACCAGTCTCCATA	50.0	47.6	58.2	57.6	451 bp	1.0	67.9
ndhG-ndhI_p5	CCCAGCGAAAAGGCAGAATA	TTGAGTCGGTTACCAGTCTC	50.0	50.0	58.2	56.6	454 bp	1.0	67.8