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rps8-rpl14

LSC · 75376-75515 · 140 bp

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Pi 0.0143

MarkerSeek score 60.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 2
pi: 0.0143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TCAGCAATAGTGTCCTTGCC	GCGATTGCCGAAGAATTGAG	50.0	50.0	57.9	57.9	232 bp	1.0	79.0
rps8-rpl14_p2	GCAATAGTGTCCTTGCCCAT	GCGATTGCCGAAGAATTGAG	50.0	50.0	58.2	57.9	229 bp	1.0	78.1
rps8-rpl14_p3	TCAGCAATAGTGTCCTTGCC	AGGAACTCGAGTTTTTGGCG	50.0	50.0	57.9	58.8	251 bp	1.0	73.5
rps8-rpl14_p4	CAGCAATAGTGTCCTTGCCC	GCGATTGCCGAAGAATTGAG	55.0	50.0	58.9	57.9	231 bp	1.0	72.7
rps8-rpl14_p5	GCAATAGTGTCCTTGCCCAT	AGGAACTCGAGTTTTTGGCG	50.0	50.0	58.2	58.8	248 bp	1.0	72.6