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petA-psbJ

LSC · 58948-59693 · 746 bp

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Pi 0.0148

MarkerSeek score 67.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 11
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	GCTGATAGGTACTGTAACCGG	50.0	52.4	58.7	57.6	941-946 bp	1.0	65.0
petA-psbJ_p2	CGTCCAAGGCCTTTTGTTCT	GGCTGATAGGTACTGTAACCG	50.0	52.4	58.7	57.6	942-947 bp	1.0	65.0
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	ACCGGTATTCTTGTGATTGGT	50.0	42.9	58.7	57.3	925-930 bp	1.0	62.2
petA-psbJ_p4	AGGCCTTTTGTTCTTTTTCGC	GCTGATAGGTACTGTAACCGG	42.9	52.4	58.2	57.6	935-940 bp	1.0	61.0
petA-psbJ_p5	AGGCCTTTTGTTCTTTTTCGC	GGCTGATAGGTACTGTAACCG	42.9	52.4	58.2	57.6	936-941 bp	1.0	61.0