Hotspot

trnF-GAA-ndhJ

LSC · 47043-47588 · 546 bp

Back to Enneapogon

Pi 0.0128

MarkerSeek score 65.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 546
variable_sites: 7
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AAAGTTGGATAGGTTGGCCC	50.0	50.0	58.1	57.8	654-658 bp	1.0	78.7
trnF-GAA-ndhJ_p2	ATGCACAAGAAAGGGTCAGG	AAAGTTGGATAGGTTGGCCC	50.0	50.0	58.1	57.8	707-711 bp	1.0	78.4
trnF-GAA-ndhJ_p3	GCACAAGAAAGGGTCAGGAT	AAAGTTGGATAGGTTGGCCC	50.0	50.0	57.8	57.8	705-709 bp	1.0	77.4
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGATAATCATCCGCGCCTT	50.0	45.0	58.1	57.5	693-697 bp	1.0	76.7
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCCGCGCCTTAAACGTATC	50.0	50.0	58.1	58.5	684-688 bp	1.0	76.5