Hotspot

atpH-atpF

LSC · 32036-32485 · 450 bp

Back to Enneapogon

Pi 0.00897

MarkerSeek score 61.60

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 4
pi: 0.00897
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GTGGCACTAGCGCTCTTATT	CGAATGGCCAGTGAGCTAAA	50.0	50.0	58.1	58.3	534-538 bp	1.0	78.6
atpH-atpF_p2	TTATGGACTGGTTGTGGCAC	CGAATGGCCAGTGAGCTAAA	50.0	50.0	58.1	58.3	547-551 bp	1.0	78.3
atpH-atpF_p3	GTGGCACTAGCGCTCTTATT	ATTAAGCCCGAAACTCCCAG	50.0	50.0	58.1	57.6	554-558 bp	1.0	77.3
atpH-atpF_p4	GTGGCACTAGCGCTCTTATT	ACGCCAACCACTACAGTTAG	50.0	50.0	58.1	57.5	604-608 bp	1.0	77.0
atpH-atpF_p5	TTATGGACTGGTTGTGGCAC	ATTAAGCCCGAAACTCCCAG	50.0	50.0	58.1	57.6	567-571 bp	1.0	77.0