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petN-trnC-GCA

LSC · 16925-17785 · 861 bp

Back to Enneapogon

Pi 0.00583

MarkerSeek score 62.60

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 861
variable_sites: 5
pi: 0.00583
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGTCCACTTCTCCCCCATAC	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	969-981 bp	1.0	71.4
petN-trnC-GCA_p2	GTCCACTTCTCCCCCATACT	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	968-980 bp	1.0	71.4
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	910-922 bp	1.0	71.2
petN-trnC-GCA_p4	AGTCCACTTCTCCCCCATAC	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	971-983 bp	1.0	70.9
petN-trnC-GCA_p5	GTCCACTTCTCCCCCATACT	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	970-982 bp	1.0	70.9