Hotspot

trnY-GUA-trnD-GUC

LSC · 14881-15265 · 385 bp

Back to Enneapogon

Pi 0.0111

MarkerSeek score 58.90

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 4
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	AATTCTTCCTGGGTCGATGC	TGAACCTAGTTCGGGACTGA	50.0	50.0	58.0	57.7	539-564 bp	1.0	81.5
trnY-GUA-trnD-GUC_p2	AATTCTTCCTGGGTCGATGC	GCGGTGCTCTAACCAATTGA	50.0	50.0	58.0	58.6	485-510 bp	1.0	79.9
trnY-GUA-trnD-GUC_p3	GGGGACGGACTGTAAATTCG	TGAACCTAGTTCGGGACTGA	55.0	50.0	58.4	57.7	506-531 bp	1.0	79.3
trnY-GUA-trnD-GUC_p4	GGGACGGACTGTAAATTCGT	TGAACCTAGTTCGGGACTGA	50.0	50.0	57.6	57.7	505-530 bp	1.0	79.1
trnY-GUA-trnD-GUC_p5	AATTCTTCCTGGGTCGATGC	GAACCTAGTTCGGGACTGAC	50.0	55.0	58.0	57.3	538-563 bp	1.0	78.7