Hotspot

trnG-GCC-trnfM-CAU

LSC · 11833-12187 · 355 bp

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Pi 0.0171

MarkerSeek score 64.50

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 6
pi: 0.0171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GAAGGTACGGGTTCGATTCC	AGTTTTTGGCCCTACAGAGC	55.0	50.0	58.1	58.1	473-477 bp	1.0	80.4
trnG-GCC-trnfM-CAU_p2	GCGAGCGTAGTTCAATGGTA	AGTTTTTGGCCCTACAGAGC	50.0	50.0	58.1	58.1	513-517 bp	1.0	80.2
trnG-GCC-trnfM-CAU_p3	GACCCAGGCGGATATATCCT	AGTTTTTGGCCCTACAGAGC	55.0	50.0	58.1	58.1	550-554 bp	1.0	79.7
trnG-GCC-trnfM-CAU_p4	CGTAGCGAGCGTAGTTCAAT	AGTTTTTGGCCCTACAGAGC	50.0	50.0	58.2	58.1	517-521 bp	1.0	79.4
trnG-GCC-trnfM-CAU_p5	ATATAGGACGTAGCGAGCGT	AGTTTTTGGCCCTACAGAGC	50.0	50.0	58.2	58.1	525-529 bp	1.0	79.3