Hotspot

trnS-GCU-psbD

LSC · 7424-8294 · 871 bp

Back to Enneapogon

Pi 0.00233

MarkerSeek score 61.60

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 871
variable_sites: 2
pi: 0.00233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1002-1016 bp	1.0	79.6
trnS-GCU-psbD_p2	TCGAAAGTCCCAAACGATCC	GCCGGACCATCCTACAAAAA	50.0	50.0	57.9	58.2	1077-1091 bp	1.0	79.1
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	989-1003 bp	1.0	79.0
trnS-GCU-psbD_p4	TCGAAAGTCCCAAACGATCC	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1064-1078 bp	1.0	78.5
trnS-GCU-psbD_p5	CGAAAGTCCCAAACGATCCA	GCCGGACCATCCTACAAAAA	50.0	50.0	58.2	58.2	1076-1090 bp	1.0	78.2